Fig. 4

Plasma and liver acyl-carnitine profiles are altered by short-term hypoxia. A Plasma acyl-carnitines and free carnitine, n = 10 Veh normoxic, n = 8 all remaining groups. B Liver acyl-carnitines and free carnitine, n = 9 Veh normoxic, n = 8 all remaining groups. Intermediates in both panels were assessed by targeted metabolomics using liquid chromatography-mass spectrometry. Data presented is peak intensity obtained from the peak area ratio, corrected to internal standards and protein concentration and normalised using autoscaling and generalised logarithm transformation. The 2 d hypoxic exposure was defined as statistically significant across all metabolites presented through use of a two-way ANOVA plus false discovery rate correction (two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, Q = 5%, threshold p value < 0.032), followed by a post-hoc Tukey’s test (p < 0.05)