Fig. 5

Hepatic triacylglycerol (TAG) composition is altered by short-term hypoxia with suppression of TAGs associated with de novo lipogenesis. A Orthogonal partial least squares—discriminant analysis (OPLS-DA) of hepatic lipidomic profiles of Veh-treated normoxic (green) and 2 d hypoxic (red) treated rats, with each dot representing the lipidomic profile of one animal. Data was obtained from the peak area ratio of LC-MS analysis, corrected to internal standards and protein concentration and normalised using Pareto Scaling and generalised logarithm transformation. B Permutation validation of the plot in A. C S-plot corresponding to the OPLS-DA model in A. Triacylglycerol species with discriminatory values, defined as those lying 2 standard deviations from the mean, are highlighted in orange. D Total peak intensities of TAGs with chain lengths 39-53. E Total peak intensities of TAGs with chain lengths 54-62. F–L TAGs with the greatest peak intensities alongside corresponding fatty acid composition derived from fragmentation analysis where 16:0 denotes palmitic acid, 16:1 palmitoleic acid, 18:1 oleic acid, 18:0 stearic acid. Data presented in C–L are Veh-treated animals in normoxia, after 2 d hypoxia and 14 d hypoxia. Peak intensities are corrected to internal standard and protein concentration. Bar graphs are presented as mean ± SEM, * p < 0.05, ** p ≤ 0.01, *** p ≤ 0.001. n = 9 Veh normoxic, n = 8 all remaining groups.