Fig. 2

Immunostaining showing the expression of opsin 3.2 in neuronal ciliary photoreceptors. Confocal laser scanning images of whole-mount S. purpuratus larvae showing immunostaining A of opsin 3.2 and synaptotagmin B at 72 h and B of opsin3.2 and α-tubulin at 96 h. C Confocal images of co-immunostaining for opsin3.2 (green, C) and synaptotagmin B (magenta, C’), showing co-expression (C”). D Confocal images of co-immunostaining for opsin3.2 with α-tubulin (D’) showing co-localization in cell surface cilia of photoreceptors (D”). E Co-immunostaining of opsin3.2 (green) and pigment cell-specific SP1 (red) showing pigment cells located in proximity to photoreceptors. Arrowheads indicate photoreceptors (A, B, E) and cilia on photoreceptors (D, D’, D”). M, mouth; AO, apical organ; CB, ciliated band