Fig. 2

Architecture of cerebral structures in E. rowelli based on different labelling techniques. Three-dimensional reconstructions using a series of Azan-stained sections, anti-acetylated α-tubulin and anti-synapsin immunolabelling of entire brains in dorsal (top row) and ventral views (bottom row). Anterior is up in all images. Corresponding neuropil regions and fibre tracts are shown in same colours. Other protocerebral neuropil areas are displayed in grey. Inset (synapsin labelling in ventral view) shows magnified details of microglomeruli. Note that Azan staining was performed on the entire head, whereas anti-acetylated α-tubulin and anti-synapsin immunolabelling was carried out on dissected brains, resulting in slightly differing shapes of neuroanatomical structures. Not all structures were reconstructed using Azan-stained sections, such as the frontal body and small tracts and neuropils. ANP, antennal neuropil; BRC 1, BRC 2, BRC 3, brain commissure 1 to 3; CB, central body; CBA, anterior division of central body; CB-IL, inner lamina of central body; CB-OL, outer lamina of central body; CBP, posterior division of central body; CNC, circumpharyngeal nerve cord; FB, frontal body; JAN, jaw nerve; FGC, fascicles of globuli cells; LIN 1, LIN 2, lip nerve 1 and 2; LO, lobes of mushroom body; OLF, olfactory fascicle; OLG, olfactory glomeruli; OLL, olfactory lobe; ONP, optic neuropil; PDN, posterior dorsal neuropil; PHN 1, pharyngeal nerves 1; PVN, posterior ventral neuropil; PNT, ponticulus; SPC, subpharyngeal commissure