Fig. 2

Percentage of PSMs containing a b-/y-type fragment ion supported CUG codon by CUG translation. A The MS/MS data of the nine C. albicans samples were processed with a database derived from the allele-resolved SC5314 genome annotation. All PSMs containing CUG positions supported by b-/y-type fragment ions (= supported PSMs; Table 1) were collected and their distribution plotted with respect to the amino acid found at the CUG position. B The plot shows the distribution of the subset of supported PSMs covering only those CUG positions, where PSMs with at least two different amino acids at the CUG positions were found. C, D The MS/MS data of the nine C. albicans samples were processed with a database derived from the WO-1 genome annotation. PSM selection and plotting as in A and B. E, F Data from the C. dubliniensis, C. tropicalis, and M. acaciae samples for comparison. The prevalent CUG translation, serine, is found in almost all of the corresponding PSMs. The numbers of CUG translations other than serine are higher for the allele-resolved C. albicans SC5314 database analyses (A) and (B) than for the pseudo-haploid WO-1 database analyses (C) and (D) indicating strain differences and haplotype merging effects. (Mis-)translation into leucine/isoleucine is, contrary to what is expected based on a suspected 3% mischarging of \( {\mathrm{tRNA}}_{\mathrm{CAG}}^{\mathrm{Ser}} \) with leucine, no more prevalent than mistranslation into any other amino acid (B) and (D)