Fig. 10

ABA and polysaccharides composition of Arabidopsis shoots. A ABA measurement in the leaves of transgenic Arabidopsis overexpressing VIH2-3B and control plants. B Venn diagram representation for the genes differentially regulated by drought stress, and transgenic lines #Line4 and #Line6 w.r.t. respective Controls. Drought-responsive genes were shortlisted using the Cufflinks pipeline after processing the datasets for 10 days of drought stress and control. C Mapman pathway analysis using Classification SuperViewer for the genes that are commonly regulated by drought stress (SRA: SRP075287) as well as transgenic lines w.r.t. control plants (#Line4 and 6). Bold terms represent significant pathways; normed frequency > 1 signifies over-representation while < 1 signifies under-representation. D For cell-wall composition analysis, wild type Col-0, Arabidopsis overexpressing TaVIH2-3B (Line#2, 4, 5, and 6), and Arabidopsis vih2-3 representing Arabidopsis mutant defective for the expression of AtVIH2 were used. Total AG: arabinogalactan, AX: arabinoxylan and cellulose (in μg/g) were measured as indicated in “Methods.” Analyses were made in triplicates, with each experimental replicate representing at least five plants for each genotype. Vertical bars represent the standard deviation. * on the bar indicates that the mean is significantly different at p < 0.001 (#at p < 0.05) with respect to their respective control plants. E Speculative model for the working of VIH2 to impart drought resistance to plants