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Fig. 2 | BMC Biology

Fig. 2

From: Wheat inositol pyrophosphate kinase TaVIH2-3B modulates cell-wall composition and drought tolerance in Arabidopsis

Fig. 2

Enzymatic activity and analysis of the PP-InsP on PAGE. A The relative luminescence units for all reactions performed were recorded using Spectramax optical reader. The kinase reactions were performed using 50 ng of TaVIH1-KD and TaVIH2-KD purified proteins for 30 min, followed by steps mentioned in the ADP-GLO kit. B Visualization of PP-InsP products on the PAGE gel (33%). The in vitro kinase reactions were performed using 30 ng of ScVIP1-KD, TaVIH1-KD, and TaVIH2-KD purified proteins for 1 and 2 h at 28 C. The reactions were then resolved on the gels (TBE-PAGE). The photo was taken after staining by Toluidine Blue

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