Fig. 5

Drought-mimic stress for VIH2-3B Arabidopsis transgenic lines. A Reporter assays using promTaVIH2:GUS transgenic lines subjected to drought-mimic (30% PEG). Seedlings with or without treatment (control) were stained overnight in GUS staining solution and photographed using a Leica stereomicroscope at × 6.3 magnification. B Transgenic Arabidopsis and control seedlings were subjected to drought-mimic conditions with glycerol (10%) and mannitol (125 mM). Ten seedlings were used for each transgenic line for each treatment. These experiments were repeated in three experimental replicates with a similar phenotype. C Root length of treated seedlings (in mm) for all the lines. Twenty seedlings were used for the measurement of root length for each line