Fig. 6

Complementation of vih2-3 line and its characterization. A Western analysis of vih2 mutant lines (vih2-3 and vih2-4) complemented with TaVIH2-3B. Multiple transgenic lines were screened and Western was done using His-Antibody using 20 μg of total protein. Coomassie Blue stain of the total protein (lower panel) was used as a loading control. B Transgenic Arabidopsis, mutant and Col-0 seedlings were subjected to drought-mimic conditions with glycerol (5%) and Mannitol (100 mM) (for moderate mimic-drought). Eight to ten seedlings were used for each transgenic line for each treatment. C Root length in mm (n = 20). These experiments were repeated three experimental replicates with similar phenotype and ten plants each