Fig. 8

RNAseq analysis of Col-0 and #Line4 and 6. A Expression pattern (as Z-scores) of top 56 genes commonly upregulated among the transgenic lines w.r.t. Col-0(Ev) in 25-day-old seedlings. Heatmap depicts the relative expression in Col-0(Ev) and overexpressing lines of TaVIH2-3B (3 biological replicates; rep1-3). B Heatmap representing a graphical summary of the Gene Ontology (GO) classification for DEGs in #Line4 and #Line6 w.r.t. Control plants. Increasing intensities of brown and blue colors represent the comparatively low and high expression for each gene, as depicted by the color scale. Normalized expression counts were used to plotting the expression as Z-scores using heatmap. Two functions from the gplots package in R. Significantly altered GO terms were identified using the Classification SuperViewer tool; the x-axis represents the GO terms where bold terms represent significant alteration while the y-axis represents the normed frequency which when > 1 signifies over-representation while < 1 signifies under-representation