Table 3 Comparison of the relative content (%) of different types of secondary structure in Tet(X4), Tet(X2), and enzymes harboring the L282S and V329M substitution
| Â | CD analysis | FTIR analysis | ||||||
|---|---|---|---|---|---|---|---|---|
Tet(X2) | Tet(X4) | L282S | V329M | Tet(X2) | Tet(X4) | L282S | V329M | |
α-helix (%) | 32.4 | 29.3 | 31.6 | 26.9 | 17.1 | 16.4 | 15.5 | 14.9 |
310-helix (%) | – | – | – | – | 26.7 | 14.4 | 11.8 | 11.4 |
β-sheet (%) | 26.1 | 24.7 | 23.9 | 23.9 | 50 | 40.4 | 43.8 | 50.7 |
Others (%) | 41.5 | 46 | 44.5 | 49.2 | 6.8 | 28.8 | 28.9 | 23 |
Spectral deviation (RMSD) | 0.182 | 0.132 | 0.123 | 0.109 | – | – | – | – |