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Fig. 5. | BMC Biology

Fig. 5.

From: Analysis of co-isogenic prion protein deficient mice reveals behavioral deficits, learning impairment, and enhanced hippocampal excitability

Fig. 5.

PrnpZH3/ZH3 mice are more susceptible to KA-induced epilepsy correlating with increased neuronal death in CA1 and CA3 pyramidal layers. a Percentage of mice reaching stage I–IV, V, or VI epileptic phenotype after KA administration (10 mg/kg). b Number of seizures and blinking episodes presented by Prnp+/+ and PrnpZH3/ZH3 mice for 3 h after KA administration. c–h Photomicrographs showing the pattern of neurodegeneration with Fluoro-Jade B staining seven days after KA treatment in Prnp+/+ (ce) and PrnpZH3/ZH3 (f–h) mouse hippocampus. Nuclei are stained with DAPI (c, f). Dying cells (d, g, stained with Fluoro-Jade B) are located in the pyramidal cell layer of CA1 (arrows) and CA3 (arrowheads) areas. i Graph illustrating the analysis of the CTCF values in the CA1-3 pyramidal layer of PrnpZH3/ZH3 and wild-type mice (see “Methods” for details). Data are presented as a percentage in a and as mean ± S.E.M, in b and i; *p < 0.05 and ***p < 0.001, Mann–Whitney U non-parametric test. Abbreviations: so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum; slm, stratum lacunosum-moleculare; DG, dentate gyrus. The scale bar in c is also representative for d–h. Source data and individual data values are available in Additional file 10

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