Fig. 6.
Reduced bursting and network formation in neuronal PrnpZH3/ZH3-derived cultures. a Immunoblot analysis of PrPC expression and PSD95 in Prnp+/+ and PrnpZH3/ZH3 derived primary neuronal cultures after 8, 11, and 15 DIV. Note the absence of PrPC in the PrnpZH3/ZH3 cultures and the same PSD95 expression in each DIV. Actin is used as a loading control. b Representative examples of neuronal traces at 8 and 15 DIV in the Prnp+/+ and PrnpZH3/ZH3 primary cultures. Note the asynchrony in the PrnpZH3/ZH3 culture. c Evolution of network bursting in Prnp+/+ and PrnpZH3/ZH3 neuronal cultures from 8 to 15 DIV. Data are presented as the mean of bursts/min ± S.E.M. d Evolution of size of synchronous bursts from 8 to 15 DIV. Data are presented as the mean percentage of active neurons ± S.E.M. Asterisks (*) indicate significant differences between Prnp+/+ and PrnpZH3/ZH3 bursting. Number sign (#) indicates significant differences with the respective baseline bursting at 8 DIV. *p < 0.05, ***p < 0.001 and ###p < 0.001, two-way ANOVA + Bonferroni’s multiple comparisons test. Source data and individual data values are available in Additional file 10