Fig. 4

Knockout of dmrt1 resulting in female development in fighting fish. A Proportion of wildtype and mutant alleles in the four screened CRISPants at both exon 1 and exon 2, where no mutant alleles were detected at exon2 for CRISPant 1, 2, and 3. B Genotypes of the four CRISPants, and four XY controls that showed no detectable mutant alleles at both exon 1 and exon 2, and XX control, determined by PCR assays using the 180-bp InDel in the SD locus. C Gonad differentiation of the four CRISPants at 13, 14, 17, and 18 dpf, respectively, in comparison to four randomly collected XY controls without detectable mutant alleles at 15, 15, 18, and 20 dpf, respectively, where arrow indicates the position of oocytes. Solid triangles indicate undifferentiated gonads. ND, nephric duct. Scale bars, 10 μm