Fig. 3.

Vimentin intermediate filaments associate with the nucleus of interphase ES cells. Representative 3D images of ES cells expressing H2B-mCherry (red) and GFP-vimentin (green). A Yellow and white arrows point to vimentin knot and ring-like structures, respectively. B Images of the cells expressing relatively low (top panels) or intermediate (bottom panels) levels of GFP-vimentin showing a close association between vimentin structures and the nucleus (Additional file 9: Supplementary Video S6 and Additional file 10: Supplementary Video S7). These images were segmented as described in Methods (middle and right panels) to facilitate their observation (scale bars: 5 μm)