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Fig. 4. | BMC Biology

Fig. 4.

From: Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells

Fig. 4.

The cytoskeletal networks regulate the nuclear morphology of ES cells. A Representative 3D image of a region of an YPet-OCT4 ES cell colony. The nuclei images were segmented to quantify the volume of each nucleus and its sphericity as described in the “Methods” section. B Quantification of the nuclei volume and sphericity in untreated YPet-OCT4 ES cells (CYPet-OCT4) or YPet-OCT4 ES cells incubated with latrunculin-B (lat), taxol (tax), or vinblastine (vbl). C Similar quantifications performed in W4 ES cells only expressing H2B-mCherry (CW4) or co-expressing H2B-mCherry and the dominant negative vimentin mutant GFP-(vim(1-138)). The data is presented as median ± SE for each experimental condition (nCYPet-OCT4 = 165; nlat = 55; ntax = 58; nvbl = 146; nCW4 = 32; nvim(1-138) = 55). Please, notice that the values measured in CYPet-OCT4 and CW4 conditions could be different due to the different emission spectra of the fluorescent protein used in each case. Asterisks indicate significant differences (p < 0.05) with respect to that obtained for the corresponding control cells. Raw data can be found in Additional file 17: Supplementary Table S2

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