Fig. 5.

OCT4-chromatin interactions are modulated by the actin and vimentin networks. Single-point FCS measurements were run in YPet-OCT4 ES cells. Mean, normalized ACF obtained at the nucleoplasm of control (gray), vimentin disrupted (A, green), and latrunculin-treated (B, orange) cells. C, D The ACF data were fitted with Eq. 1 to obtain the fractions of free (diffusion), long-lived bound and short-lived bound TF molecules (C) and the characteristic times of long-lived and short-lived interactions of the TF with chromatin (D). The data is presented as mean ± SE for each experimental condition (control: gray bars, n = 17; vim(1-138): green bars, n = 17; latrunculin-B: orange bars, n = 12). Asterisks denote significant differences (p < 0.01) with respect to the control condition. Raw data can be found in Additional file 18: Supplementary Table S3