Fig. 3

Validation of RNA-sequencing dataset and SCPP repertoire gene expression during scale regeneration. A Comparison of relative expression levels of qRT-PCR (unpaired t test) and transcriptomic analysis (false discovery rate) of selected amplicons. Total RNA used for RNA-seq experiment was used. B Quantitative real-time PCR (qRT-PCR) of secretory calcium-binding protein (SCPP) family genes that were differentially expressed in the RNA-seq dataset (n = 4 fish). Two-way ANOVA analysis showed that there was no interaction factor variance (p = 0.882) but that time (f(4) = 11.05, p=0.001) was the only independent statistically significant effect on amplicon expression