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Fig. 3 | BMC Biology

Fig. 3

From: Long-term in vivo application of a potassium channel-based optogenetic silencer in the healthy and epileptic mouse hippocampus

Fig. 3

Spectral analysis reveals reduced power of gamma oscillations during light activation of PACK. A Representative spectrogram snippets from a ref. recording show baseline activity; blue colors reflect low power and red colors reflect high power of the corresponding frequencies. B Mean power spectral density (PSD) during the first (light gray), second (dark gray), and third (black) recording hour. C Light pulses applied at 0.1 Hz transiently decreased the spectral power. D The mean PSDs pre- (light gray), light ON (blue), and post-recordings (black). EG The oscillatory power was calculated by taking the AUC of the PSD plot in each frequency range: delta (1–4 Hz), theta (4–12 Hz), beta (12–30 Hz), and gamma (30–120 Hz). During the Eref. recordings, the power of theta, beta, and gamma oscillations declined significantly. F During 0.1-Hz illumination, the power of beta and gamma oscillations also decreased notably. Two-way RM ANOVA, Dunnett’s multiple comparison test (n = 6 mice, average of two recordings, gray lines). G Power change from the first to the second hour in ref. versus 0.1-Hz recordings revealed that only gamma power was further reduced by the light-induced PACK activation. Multiple paired t-tests with an adjusted significance level (α = 0.0125). Mean presented in black with SEM as error bars

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