Fig. 6
From: The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1
Side-chain property at position 572. a Representative whole-cell recordings from HEK293 cells expressed TRPV1(M572A), TRPV1(M572I), or TRPV1(M572L) evoked by 1,4-dioxane (3% and 5%), 1,3-dioxane (1% and 2%), tetrahydrofuran (THF, 1% and 2%), and capsaicin (Cap, 1 μM). Holding potential was −60 mV. b Averaged responses of the mutant TRPV1 channels evoked by different stimuli as indicated. Current densities were determined by dividing the peak current amplitudes by membrane capacitance. c Concentration-response curves of mutant TRPV1 channels. Solid lines are fits to Hill’s equation. For 1,4-dioxane, EC50 = 4.64 ± 0.24%, and nH = 4.03 ± 0.75 for V1(M572A) (n = 9); EC50 = 4.83 ± 0.24%, and nH = 4.33 ± 0.90 for V1(M572I) (n = 6); EC50 = 2.96 ± 0.13%, and nH = 4.26 ± 0.76 for V1(M572L) (n = 8). For 1,3-dioxane, EC50 = 3.11 ± 0.06 %, and nH = 6.88 ± 0.96 for V1(M572A) (n = 6); EC50 = 3.02 ± 0.04%, and nH = 6.22 ± 0.55 for V1(M572I) (n = 5); EC50 = 1.36 ± 0.05%, and nH = 6.25 ± 1.02 for V1(M572L) (n = 7). For tetrahydrofuran, EC50 = 1.49 ± 0.02%, and nH = 5.08 ± 0.15 for V1(M572A) (n = 5); EC50 = 1.65 ± 0.01%, and nH = 4.97 ± 0.12 for V1(M572I) (n = 5); EC50 = 0.99 ± 0.01%, and nH = 4.73 ± 0.35 for V1(M572L) (n = 5)