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Fig. 5 | BMC Biology

Fig. 5

From: The visual pathway in sea spiders (Pycnogonida) displays a simple serial layout with similarities to the median eye pathway in horseshoe crabs

Fig. 5

Histamine immunolabeling in eyes and first-order visual neuropils of E. spinosa. A 3D-reconstructed volume rendering of protocerebral sense organs and their connection to the brain, μCT scan, lateral view. Stippled brackets indicate the positions of the extended sections shown in BE. B–D Tubulin (TUB, magenta, shown in the left row of images) and histamine (HIS, green) immunolabeling with nuclear counterstain and cuticular autofluorescence (AF + NUC, gray), horizontal sections (MIP) through the ocular tubercle arranged from distal (B) to proximal (D). Note strong autofluorescence in the cuticular lenses and the cuticle rim of the LO. The R-cells are strongly histamine-ir, while the cells in the LO are devoid of histamine signal. E Histamine immunolabeling (green) with nuclear counterstain (gray, only upper image), horizontal section (MIP) through the anterior brain region. Note strong histamine labeling of R-cell axon terminals in the visual neuropils but absence of signal in the median brain region housing the antero-median neuropil. Abbreviations: AE – anterior eye; BR – brain; LO – lateral sense organ; LONV – lateral sense organ nerve; LT – lateral thickening; PE – posterior eye; RTA – R-cell axons; VN – visual neuropil

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