Fig. 6

DiI backfills from eyes and lateral sense organ in E. spinosa. Extended sections (MIP) of DiI backfills (green) with nuclear counterstain and cuticular autofluorescence (AF + NUC, gray). Counterstain not shown in lower (A,E) and right (B–D,F,G) images. A–D Backfills from the anterior (B–D) and posterior (A) eyes. A Para-sagittal section of ocular tubercle and brain. Note absence of DiI signal in the anterior eye and the lateral sense organ. Stippled brackets indicate the positions of the extended sections shown in C and D. B–D The black arrowhead marks diffuse labeling in the proximal portion of the tract to the arcuate body. The antero-median neuropil is unlabeled. B Para-sagittal section. The yellow and red arrowheads point to weak signal in the optic nerve branches to the antero-median neuropil and the central brain neuropil, respectively. C Horizontal section. Note stronger labeling in one half of the visual neuropil. D Cross section. E–G Backfills from the lateral sense organ. The visual neuropil is unlabeled. Yellow arrowheads mark neurites in the optic nerve branch projecting to the antero-median neuropil. Black arrows indicate single projections from the antero-median neuropil into the central brain neuropil. White arrows point to a labeled spherical body in the cortex. The asterisk marks a weakly labeled projection toward the contralateral lateral thickening. E Para-sagittal section of ocular tubercle and brain. The arrowhead points to neurites of the subepidermal network surrounding the lateral sense organ. Stippled brackets indicate the positions of the extended sections shown in F and G. F Horizontal section. G Cross section. Abbreviations: ABU – upper arcuate body layer; AEL – anterior eye lens; AMN – antero-median neuropil; BRN – brain neuropil; LO – lateral sense organ; LONV – lateral sense organ nerve; LT – lateral thickening; ONV – optic nerve; PEL – posterior eye lens; RTA – R-cell axons; VN – visual neuropil