Table 2 Antibodies/antisera, research resource identifiers and dilutions

Primary

Rabbit anti-5-hydroxytryptamin (serotonin), polyclonal, ImmunoStar, #20080

AB_572263

1:1000

Rabbit anti-histamine, polyclonal, Progen, #16043

AB_2892841

1:1000

Rabbit anti-Asn¹³-orcokinin, polyclonal, gift from Heinrich Dircksen (Stockholm University)

AB_2315017

1:1500

Rabbit anti-A-type Dip allatostatin I, polyclonal, Jena Bioscience, #ABD-062

AB_2314318

1:2000

Rabbit anti-proctolin, polyclonal, Jena Bioscience, #ABD-032

AB_2892840

1:1000

Mouse anti-tyrosine hydroxylase, IgG1 isotype, clone LNC1, ImmunoStar, #22941

AB_572268

1:300

Mouse anti-SYNORF1 (Drosophila synapsin-1), monoclonal, supernatant, Developmental Studies Hybridoma Bank, #3C11

AB_528479

1:100

Mouse anti-acetylated tubulin IgG 2b isotype, clone 6-11 B-1, Sigma-Aldrich, #T6793

AB_477585

1:200–300

Rat anti-alpha-tubulin IgG2a isotype, clone YL1/2, Thermo Fisher Scientific, #MA1-80017

AB_2210201

1:500

Secondary

Alexa Fluor488 goat anti-mouse IgG (H + L), polyclonal, Thermo Fisher Scientific, #A11001

AB_2534069

1:250

Cy3-AffiniPure goat anti-mouse IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #115-165-166

AB_2338692

1:250

Alexa Fluor647-AffiniPure goat anti-mouse IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #115-605-166

AB_2338914

1:250

Alexa Fluor488-AffiniPure goat anti-rabbit IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #111-545-144

AB_2338052

1:250

Alexa Fluor594 goat anti-rabbit IgG (H + L), polyclonal, Thermo Fisher Scientific, #A11012

AB_2534079

1:250

Alexa Fluor647-AffiniPure goat anti-rat IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #112-605-167

AB_2338404

1:250

unconjugated AffiniPure Fab fragment donkey anti-rabbit IgG (H + L), polyclonal, Jackson ImmunoResearch Labs, #711-007-003

AB_2340587

1:25