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Fig. 1 | BMC Biology

Fig. 1

From: CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles

Fig. 1

LentiFlash® particles allow the highly efficient delivery of RNA molecules in hiPSC. a Fluorescent microscopy analysis of HY03 hiPSC 48 h post-transduction with LF-ZsGreen particles at 0.5, 2, or 5 pg p24/cell. b Transduction efficacy by flow cytometry analysis of ZsGreen-positive HY03 cells at 48 h post-transduction, and c mean fluorescent intensity (MFI) fold change assessed by cytometry analysis at 48 h post-transduction (same conditions as in a). d Fold change of lactate dehydrogenase (LDH) concentration in HY03 cell supernatant samples collected at 24, 48, and 72 h post-transduction (same conditions as in a); NT, not transduced cells (n = 1); ns, not significant; **P < 0.005; ***P < 0.0005. e Cell number at 24, 48, and 72 h post-transduction (same conditions as in a); NT, not transduced cells (n = 1); ns, not significant. f Fold change of ZsGreen mRNA level in HY03 cells in function of time. The sample corresponding to cells transduced with 5 pg p24/cell and analyzed at 48 h was arbitrarily defined as the reference (=1) (same conditions as in a, n = 1). g Flow cytometry analysis of ZsGreen-positive cells for 10 days after transduction (NT, not transduced cells)

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