Fig. 3

Viral infection of porcine PSCdMs. A Bright-field image from a haemadsorption assay of ASFV-infected porcine PSCdMs, 24 h post-infection. Red blood cells can be seen aggregating around two PSCdMs (black arrows). B Composite bright-field and fluorescent image of ASFV-infected PSCdMs, 24 h post-infection, stained for p72 viral protein (green). C RT-qPCR analysis of ASFV levels (genome copies) present in supernatants from PAMs and PSCdMs 24 h and 48 h post infection. Mean and SD of four experimental replicates. D Flow cytometry analysis of cell surface CD163 (green) on PAMs and PSCdMs derived from two independent PSC lines relative to isotype control (red) and unstained cells (grey). E Confocal image of a PRRSV-infected PSCdM, 19 h post-infection and stained for PRRSV nucleocapsid protein (green). DNA is stained with DAPI (blue) and actin filaments with phalloidin (red). F Flow cytometry analysis for PRRSV nucleocapsid protein in PRRSV-infected PAMs (blue) and two independent PSCdM lines (red) 18 h post-infection and relative to uninfected PSCdMs (grey)