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Fig. 1 | BMC Biology

Fig. 1

From: Epigenetic regulation of 5α reductase-1 underlies adaptive plasticity of reproductive function and pubertal timing

Fig. 1

Pre-pubertal colitis in mice affects pubertal timing and ovarian function. A Age of vaginal opening (VO), indicating onset of puberty. ***P < 0.001; n = 47, 59. B Age of VO in second generation, relative to that in off-spring of parent littermate controls. P > 0.05; n = 20, 30. C Ovulatory rates (average number of successful pregnancies for each mouse) and D litter sizes in DSS-treated (n = 14 litters) and littermate control mice (n = 20 litters) from 5 mice in each treatment group after four encounters with the same male; *P = 0.024 (Mann-Whitney t-test). E Circulating AMH (ng/ml) in control [n = 11] and DSS-treated [n = 14] mice, **P = 0.008. FI H&E stained ovarian histological sections from F, G control and H, I DSS-treated groups. Some atretic follicles (yellow asterisks) and zona pellucida remnants (green arrows) are marked. J Follicle counts from sections of mice ovaries (n = 4, n = 6), compared by t-test. K Volcano plot and L heat map of differentially expressed ovarian genes at Padj < 0.05 from RNA-seq analysis. M Signaling pathway to follicle activation, showing some of the differentially expressed genes (DEGs; P < 0.05). Red boxes signify upregulated genes; green boxes signify downregulated genes

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