Fig. 2

Srd5a1 is downregulated and hypermethylated in mice and women following early-life challenge. A Differentially methylated regions associated with three genes (Illumina EpicMethylation sites) in buccal DNA of Bangladeshi women who grew up in Bangladesh (n = 16) or UK (n = 13); mean ± SEM. B The mRNA levels of the same three genes in control (n = 16) and DSS-treated (n = 14) mice ovaries from the RNA-seq analysis; ***Padj < 0.001; mean ± SEM. C Confirmatory qPCR analysis of the Srd5a1 mRNA levels (n = 5), **P = 0.007; showing means with individual data points. D Levels of CpG methylation in the 5’region of the Srd5a1 first intron (corresponds with first site in Fig. 2A: see Fig S4), in control (n = 24) and DSS-treated (n = 29) mice ovaries, mean ± SEM shown relative to controls; **P = 0.015 (Mann-Whitney t-test). E Correlation between the levels of Srd5a1 mRNA (from RNA-seq analysis) and methylation measured in the same samples; P = 0.0085