Fig. 4

PyrNs exhibit binocular responses. A Schematic overview of left and right stimulus presentation experiment. B StDev projection image of a timeseries acquired from a id2b:gcamp6s larva during ramp stimulus presentation to the left eye. Note the presence of active PyrNs in both the contralateral OT (black arrowheads) and ipsilateral OT (red arrowheads). C StDev projection image of same tectum as B during 10s ramp stimulus presentation to the right eye. Note the presence of active PyrNs in both the contralateral OT (black arrowheads) and ipsilateral OT (red arrowheads). D Schematic overview of stimulus presentation to the right eye of enucleated larvae. Lower panel is a transmitted light image of an 8dpf larva in which the left eye was surgically removed at 3dpf. E StDev projection image of a timeseries acquired from a left eye-enucleated larva during 10s ramp stimulus presentation to the right eye. F Ramp-evoked responses in contralateral neurons (c1-3) and ipsilateral neurons (i1-3) of tectum shown in E. Note that all six neurons exhibit consistent responses to each ramp stimulus cycle. G Response class distribution in left tectum (normal retinal input) and right tectum (no retinal input) of left eye-enucleated larvae. N = 26 and 19 neurons from 9 larvae for each condition, two-way ANOVA, interaction effect p=0.985, with posthoc unpaired t test. H, I Comparison of response onset (LUX at half max) and response duration (LUX at half max) in the left and right tectum of left eye-enucleated larvae presented with 10s ramp stimuli. Two-way ANOVA, interaction effects p=0.607 and 0.946, with posthoc unpaired t test. Note: for t test comparisons in G–I only p values that reached significance are shown. Scale bar: 50μm in B, C, and E