Fig. 6

TL is required for PyrN binocularity. A Maximum projection image of a 6 dpf Tg(vglut2a:dsred, id2b:gcamp6s) double transgenic larva prior to laser ablation of TL. B Larva in A at 7dpf, 1 day following targeted laser ablation of TL at 6 dpf. Note normal brain structure and similar density of GCaMP6s+ PyrNs in OT. C Sideview images of green-boxed regions in A and B. Note reduction in vglut2a:dsred+ cells in TL ablated larva. Graph at right depicts quantification of TL ablation using area measurements obtained from sideview projections. N = 6 larvae, paired t test. D Single confocal images at Z-planes through dorsal TL indicated by blue lines in C. Note strong reduction in vglut2a:dsred+ cells in TL ablated larva. E StDev projection image of timeseries acquired from a left eye enucleated larva with intact TL. Black arrowheads denote active neurons in contralateral OT with intact retinal input, red arrowheads denote active neurons in ipsilateral OT devoid of retinal input. Note similar proportion of active PyrNs in contra and ipsi OT. F StDev projection image of timeseries acquired from a left eye enucleated and TL-ablated larva. Black arrowheads denote active neurons in contralateral OT with intact retinal input, red arrowheads denote active neurons in ipsilateral OT devoid of retinal input. Note reduced number of active neurons in ipsilateral OT compared to contra OT. G Percentage of active neurons located in right/ipsilateral tectum during stimulation of right eye in enucleated larvae and larvae that underwent both enucleation and TL ablation. n=21 and 20 larvae, p=0.0033, unpaired t test. H Quantification of active PyrNs (expressed as a percentage of total id2b+ neurons) in ipsilateral vs. contralateral tectum in left eye-enucleated larvae during 10s ramp presentation to right eye. N=10 larvae, paired t test. I Quantification of active PyrNs (expressed as a percentage of total id2b+ neurons) in ipsilateral vs. contralateral tectum in larvae that underwent both enucleation and TL ablation during 10s ramp presentation to right eye. Note strong reduction in active PyrNs in all but one larva. N=8 larvae, paired t test. Scale bar: 75μm in A–B, 50μm in C–D, and 60μm in E–F