Fig. 2From: Highly reliable creation of floxed alleles by electroporating single-cell embryosSchematics of 2-round targeting. A male with a deletion allele and an allele with loxP insertion in site 1 and an indel at site 2 (Target 3). Sperm from the male is used for IVF of wild type oocytes, and a new gRNA/Cas9 (RNP3) is electroporated with ssODN2 to insert the loxP specifically into Target 3. All resulting animals carrying both loxP sites have a floxed alleleBack to article page