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Table 1 Successful floxed projects

From: Highly reliable creation of floxed alleles by electroporating single-cell embryos

Target

Floxed distance (bp)

Embryos transferred

Live births

Pups with both loxPs

Floxed/bred

Abat

6614

492

62

6

1/3

Amdhd1

1935

598

138

2

1/2

Ar

3237

220

32

3

1/3

Atg5

4955

542

85

3

1/2

Atpif1

1207

327

104

7

1/2

Batf3

4151

435

70

2

1/1

Bptf

1126

214

24

2

1/2

Dcaf12

1273

619

142

1

1/1

Fcgr3

3225

426

91

7

2/5

Gfpt1

2619

430

44

2

1/2*

Gfpt2

1523

450

75

2

1/2*

Glut8

658

318

83

8

2/4

Gnpat

10737

660

92

4

1/4

Gpd2

825

422

124

2

1/2

H2-Dma

3760

613

160

7

3/7

Hal

1269

488

100

4

1/1

Ifi35

1273

467

91

11

2/3

Igh

988

483

86

5

2/3

Il4

6614

414

119

3

1/2*

Irgm1

2405

424

135

1

1/1

Itgax

1075

492

94

5

1/5

Kmt2c

1880

479

111

6

4/4

Ldlrad3

160942

534

125

4

1/3

Lrp1

1167

548

70

3

1/2

Lrpap1

448

315

89

3

1/2*

Mmp14

1822

447

66

4

2/4

Mxra8

5408

466

104

3

1/2

Nat10

1880

558

127

7

3/3

Nptn

1592

548

94

4

1/4

Per1

1920

430

81

3

1/2*

Sarm1

1218

483

101

3

1/2*

Slpi

1283

402

95

7

1/3*

Skida

3144

623

86

5

2/2*

Stmn2

1208

506

83

5

1/2*

Tcf19

2735

552

70

10

1/3*

Tifa

3002

581

117

5

1/2

Uba5

1177

438

52

3

1/3

Ube4a

9908

457

97

9

3/4

Ufsp2

632

449

45

2

1/2

Xylt1

7779

386

116

12

2/3

  1. Floxed/bred refers to number of germline-confirmed floxed founders over the number of founders with both loxP sites that were bred to F1 generation
  2. *Projects tested using in vitro Cre recombination assay. Positive Cre assay results correlated to successful germline transmission