From: Highly reliable creation of floxed alleles by electroporating single-cell embryos
Target | Floxed distance (bp) | Embryos transferred | Live births | Pups with both loxPs | Positive/tested for in vitro Cre assay |
---|---|---|---|---|---|
Flt4 | 719 | 556 | 59 | 1 (1.7%) | 1/1 |
Ccr7 | 2412 | 575 | 151 | 2 (1.3%) | 2/2 |
Tmem135 | 726 | 555 | 166 | 11 (6.7%) | 6/11 |
Dsg2 | 1649 | 265 | 38 | 3 (7.9%) | 1/3 |
Prf1 | 2642 | 445 | 69 | 4 (5.7%) | 4/4 |
Ifng | 1547 | 483 | 70 | 3 (4.3%) | 3/3 |