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Table 3 Genotypes of F0 male candidates suitable for retargeting

From: Highly reliable creation of floxed alleles by electroporating single-cell embryos

Target 5′ end 3′ end
Total Reads Allele #1 Allele #2 Total Reads Allele #1 Allele #2
Nmnat2, F0 501 −2 (95.8%) −3 (3.6%) 628 loxP (100.0%)  
Scn5a, F0a 1601 −27 (94.9%) −15 (3.6%) 1975 WT (2.2%) loxP (88.9%)
Slc38a9, F0 890 loxP (99.9%)   1624 −2 (92.2%)  
Zfhx4, F1 1210 WT (99.2%)   990 WT (56.7%) loxP (42.5%)
Zfhx4, F0 193 loxP (97.4%)   154 −6 (99.4%) −4 (0.6%)