Table 3 Genotypes of F0 male candidates suitable for retargeting
From: Highly reliable creation of floxed alleles by electroporating single-cell embryos
| Target | 5′ end | 3′ end | ||||
|---|---|---|---|---|---|---|
| Total Reads | Allele #1 | Allele #2 | Total Reads | Allele #1 | Allele #2 | |
| Nmnat2, F0 | 501 | −2 (95.8%) | −3 (3.6%) | 628 | loxP (100.0%) | |
| Scn5a, F0a | 1601 | −27 (94.9%) | −15 (3.6%) | 1975 | WT (2.2%) | loxP (88.9%) |
| Slc38a9, F0 | 890 | loxP (99.9%) | 1624 | −2 (92.2%) | ||
| Zfhx4, F1 | 1210 | WT (99.2%) | 990 | WT (56.7%) | loxP (42.5%) | |
| Zfhx4, F0 | 193 | loxP (97.4%) | 154 | −6 (99.4%) | −4 (0.6%) | |