Table 4 Completed retargeting projects. All have transmitted the floxed allele. The NA designates “not applicable” to those targets where allele-specific gRNAs were used, and no Cre assay was performed
From: Highly reliable creation of floxed alleles by electroporating single-cell embryos
Target | Live births | Pups with both loxPs | Positive/tested for Cre assay | Germline transmission confirmed |
|---|---|---|---|---|
Nmnat2 | 124 | 22 | NA | Yes |
Slc38a9 | 32 | 3 | NA | Yes |
Zfhx4 | 40 | 2 | 1/2 | Yes |