Table 5 gRNA3 sequences used for retargeting. NGG, the PAM site following 20 bp of spacer sequence for each gRNA. Red dashes, --, represent deleted bases from the wild type spacer sequences and upstream bases added at the 5′ end of the spacer sequences are in red. Zfhx4 was retargeted using the original gRNA against the wild type target. All gRNAs were ordered from IDT as one-piece synthetic gRNAs
From: Highly reliable creation of floxed alleles by electroporating single-cell embryos
Target | Initial gRNA | gRNA targeting indel |
|---|---|---|
Nmnat2 | 5′-TCAAGCAACGGTAATGCTGCNGG | 5′-TCTCAAGCAACGGTAATGC--CNGG |
Slc38a9 | 5′-GTCCTGTGCGGCCTTGTATTNGG | 5′-TTGTCCTGTGCGGCCTTG--TTNGG |
Zfhx4 | 5′-TAATTAGGCCGACATGAACGNGG | 5′-TAATTAGGCCGACATGAACGNGG |