Fig. 1

Metabolic reprogramming after NAA40 depletion in AML12 hepatocytes. A RT-qPCR analysis of expression of NAA40 mRNA levels in mock, scramble, and NAA40-KD cells after 48 h of siRNA treatment (n = 4/group). B Representative immunoblots (left) of protein extracts run in quadruplicate using antibodies against NAA40 and β-actin as loading control as well as western blot analysis of histone extracts using antibodies against the NAA40 antagonistic mark H2A/H4S1ph and H3, H2A, and H4 as loading controls in mock, scramble, and NAA40-KD cells after 48 h of siRNA treatment. Right plot indicates quantification of immunoblot signals NAA40 relative to β-actin. C Aqueous metabolites obtained by LC-MS 48 h after siRNA treatment were subjected to Metaboanalyst for enrichment and pathway analysis. D Analysis of the indicated TCA cycle intermediates in mock, scramble, and NAA40-KD cells measured by LC-MS after 48 h of siRNA treatment, with acetyl-CoA having the most significant changes (n = 4/group). E Schematic representation of the synthesis and consumption of cytosolic acetyl-CoA in mammalian cells. F Representative immunoblots of histone extracts run in triplicate using antibodies against the indicated histone acetylation marks and H3/H4 as loading controls in mock, scramble, and NAA40-KD cells after 48 h of siRNA treatment (n = 3/group). G RT-qPCR analysis of DNL synthesis genes Acly, Acaca, Fasn; triglyceride synthesis genes, Gpat1, Agpat1, Pap and Dgat1 and breakdown genes, Hsl and Atgl after 48 h of siRNA treatment (n = 4/group). All data are presented as mean ± SEM and analysed by 2-way ANOVA with post hoc Tukey’s multiple-comparisons test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Individual values can be found in Additional file 9: Table S3