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Fig. 3 | BMC Biology

Fig. 3

From: Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis

Fig. 3

NAA40 affects accumulation of lipid droplets, the insulin pathway, and glucose uptake. A Schematic representation of acquired insulin resistance from accumulated lipid droplets. Illustration was created with BioRender.com. B Representative image of lipid droplets by Nile red (red) and nuclei by DAPI (blue) in scramble and NAA40-KD cells with and without FSG67, 48 h after siRNA treatment; Scale bar = 25 μm. Right plot represents the quantification of Nile red using ImageJ (n = 8–10/group). C Representative immunoblot (right) run in triplicate of cell extracts using antibodies against pAKTser473, total AKT, and β-actin as loading control in scramble and NAA40-KD cells with and without FSG67, 48 h after siRNA treatment. Right plot indicates quantification of immunoblot signals pAKT-ser473 relative to total AKT (n = 3/group). D Representative images (top) and histograms (bottom) show comparative glucose uptake between scramble and NAA40-KD cells with and without FSG67, after 48 h of siRNA treatment; cells were incubated with 2-NBDG in glucose free media for 30 min. Right plot shows mean fluorescence uptake quantification in scramble and NAA40-KD cells with and without FSG67, after 48 h of siRNA treatment (n = 4/group). E Representative image of lipid droplets by Nile red (red) and nuclei by DAPI (blue) in scramble and NAA40-KD cells with and without insulin or insulin lispro, 48 h after siRNA treatment; Scale bar = 25 μm. Right plot represents the quantification of Nile red using ImageJ (n = 7–10/group). F Representative immunoblot (right) run in triplicate of cell extracts using antibodies against pAKTser473, total AKT, and β-actin as loading control in scramble and NAA40-KD cells with and without insulin or insulin lispro, 48 h after siRNA treatment. Right plot indicates quantification of immunoblot signals pAKT-ser473 relative to total AKT (n = 3/group). Data are presented as mean ± SEM and analysed by 1-way ANOVA with post hoc Dunnett’s multiple-comparisons test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Individual values can be found in Additional file 9: Table S5

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