Fig. 4

Metabolic rewiring upon NAA40 depletion precedes potential transcriptional effects of NAA40. A Visualisation (right) and quantification (left) of neutral lipids by Nile red (red) and nuclei by DAPI (blue) in scramble and NAA40-KD cells, 6, 12, 24, and 48 h of siRNA treatment; Scale bar = 25 μm (n = 6/group). B Representative immunoblot of whole cell extracts run in triplicate using antibodies against NAA40 and β-actin, as well as western blot analysis of histone extracts using antibodies against the indicated histones and H2A/H4S1 phosphorylation mark (n = 3/group). C Relative abundance of H2A/H4S1ph measured by western blots as well as of lipid droplets measured by Nile red staining 6, 12, 24, and 48 h of siRNA treatment. D RT-qPCR analysis of Naa40 and DNL synthesis genes Acly, Acss2, Acaca, and Fasn after 12 h of siRNA treatment (n = 3/group). E Visualisation (right) and quantification (left) of neutral lipids by Nile red (red) and nuclei by DAPI (blue) in scramble, NAA40-KD and NAA40-KD + Actinomycin D cells for 12 h; Scale bar = 25 μm (n = 6–8/group). Data are presented as mean ± SEM and analysed by 1-way ANOVA with post hoc Dunnett’s multiple-comparisons test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Individual values can be found in Additional file 9: Table S6