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Fig. 1 | BMC Biology

Fig. 1

From: A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth

Fig. 1

CRISPR/Cas9-mediated double gene mutation of PxABCC2 and PxABCC3 in P. xylostella. a The contiguous genomic structure of PxABCC2 and PxABCC3. Exons are shown as boxes except for the first and last exons which are represented by triangles and arrows respectively. The sgRNA target sequences of PxABCC2 and PxABCC3 are highlighted by green text, the PAM sites are highlighted in red and underlined. The remaining sequences after cleavage are shown in blue. b Protein structures of PxABCC2 (right) and PxABCC3 (left), as well as the cleavage position following editing. c Genotyping of individual P. xylostella for deletion of PxABCC2 and PxABCC3 according to the banding profile of PCR products from the four primer pairs. d Representative chromatograms of direct sequencing of PCR products with the primer pair 3-2-F/2-3-R, the arrow indicates the junction of PxABCC2 and PxABCC3 after editing. In the nomenclature of these primers, the first number denotes the gene, the second number indicates the exon/intron. M: DNA marker III

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