Fig. 10
Expression and function of Twitch-2B in the granulosa cells of mouse ovarian follicles. A A follicle from a mouse expressing Twitch-2B (homozygote). A follicle consists of multiple layers of granulosa cells surrounding an oocyte in the center. Twitch-2B is uniformly expressed in the granulosa cells but is not detectable in the oocyte. A layer of theca cells, adhering to the outside of the follicle, expresses Twitch-2B at a higher level. Scale bar = 50 μm. B ~ 6-fold increase in YFP/CFP ratio in the outer granulosa cells after application of 10 μM ionomycin in the presence of 10 mM CaCl2. Representative of n = 4 follicles