Fig. 5

Genome-wide analysis of DNA off-target effect for the GhABE7.10 system by whole-genome sequencing. A Sequence alignment of sgRNA2 target sites on At and Dt subgenomes with IGV. The A>G (T>C) mutations edited by GhABE7.10d was detected at the A5 site of target region. The target sgRNA sequences are reverse complementarity and highlighted in different colors. B Numbers of total SNVs identified in the GhABE7.10n, GhABE7.10d, WT, and negative plants. C Characterization of DNA off-target SNVs (A-to-G/T-to-C) in GhABE7.10n and GhABE7.10d-edited plants. The DNA off-target SNVs (A-to-G/T-to-C) are randomly distributed across the cotton chromosomes in GhABE7.10n and GhABE7.10d plants. The tracks from outer to inner circles indicate the A-to-G (blue circle) and T-to-C (red circle) SNVs that identified at DNA levels of samples GhABE7.10n, GhABE7.10d, Jin668, and Negative. D Annotation of SNVs in the intergenic, exonic, intronic, upstream, and downstream regions of two GhABE7.10-edited T0 plants