Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

Table 1 Summary of editing frequencies of GhABE-n/dCas9 and GhABE-dCpf1

Base Editor	Adenine deaminase type	Cas9	Gene target	Number of checks	T0 positive strains	T1 positive strain	T0 editing efficiency (individual)
GhABE7.10n	TadA7.10	nCas9	GhPEBP	80	62	13	2–64%
GhABE7.9n	TadA7.9	nCas9	GhPEBP	24	21		3.83–6.1%
GhABE7.8n	TadA7.8	nCas9	GhPEBP	19	13		2.26–5.5%
GhABE6.3n	TadA6.3	nCas9	GhPEBP	15	10		3.12–4.89%
GhABE7.10n	TadA7.10	nCas9	GhCLA	42	27	6	~ 12.57%
GhABE7.9n	TadA7.9	nCas9	GhCLA	22	18		~ 3.77%
GhABE7.8n	TadA7.8	nCas9	GhCLA	11	9		~ 1.06%
GhABE6.3n	TadA6.3	nCas9	GhCLA	13	10		~ 1.00%
GhABE7.10d	TadA7.10	dCas9	GhPEBP	22	18	1	1.3–7.3%
GhABE7.9d	TadA7.9	dCas9	GhPEBP	9	7		1.06–3.46%
GhABE7.8d	TadA7.8	dCas9	GhPEBP	25	21		~ 3.5%
GhABE6.3d	TadA6.3	dCas9	GhPEBP	18	18		~ 3.44%
GhABE7.10dCpf1	TadA7.10	dCpf1	GhPEBP	18	16		~ 0.45

Note: Base editing efficiency was calculated by the ratio of reads with editing in the total reads at the target region of edited plants. Comparisons are based on base editing at sgRNA2- and sgRNA5-target sites

ISSN: 1741-7007