Fig. 5

Larvicidal activity of desmethylglycitein (DMG) on Ae. aegypti. A Survival rates of the 1st instar larvae of Ae. aegypti 24 h after treatments of daidzein (DAI, black dots and lines) and DMG (magenta dots and lines). Control (0.1% ethanol without any flavonoids), and 1, 10, and 100 ppm of flavonoids were used. Each dot represents survival rates of twenty larvae in each independent experiment. Fitting curves were generated using log-logistic equation: Mortality = 1/(1+exp{b(log([flavonoid concentration])-log(LD₅₀)}); b = −0.781911 and LD₅₀ = 85.83 ppm for DAI; b = −1.081635 and LD₅₀ = 9.39 ppm for DMG. **p < 0.01 Student’s t-test. B Representative photos of control and 2.5 ppm DMG-treated Ae. aegypti larvae 24 h after treatments. Scale bar, 1 mm. C The transverse diameter of the Ae. aegypti larval head was measured 24 h after adding 2.5 ppm DMG or control 0.1% DMSO. Raw data are described in Additional file 2: Table S6. ** p < 0.01 Student’s t-test. Error bars: standard deviations. D RT-qPCR analysis of E74B mRNA level under the conditions described for C. E74B mRNA levels are normalized by rp49 mRNA levels. A mean normalized expression level of E74B is set as 1. *p < 0.05 Student’s t-test. Error bars: standard deviations