Fig. 6

AsegIR75p.1 and AsegIR75q.1 respond to medium-chain fatty acids in Xenopus oocytes. A Response profiles of AsegIR75p.1 (left) and AsegIR75q.1 (right) to the full odor panel. Response magnitudes were normalized to the average response of primary ligand (N ≥ 4). Error bars indicate the SE. B,C Upper panel: Representative current traces of oocytes upon successive exposures to 100 μM stimuli. Each compound was applied at the time indicated by the arrowheads for 20 s. Lower panel: Dose-dependent responses, showing values normalized to the average response of the most active compound at 100 μM (N ≥ 4 for each ligand). Individual data values are listed in Additional file 3. The responses of AsegIR75p.1 to the four primary ligands at each dose were compared by one-way ANOVA followed by an LSD test. * in B indicates the doses at which the responses to hexanoic acid are significantly higher than to the other three ligands (p < 0.05). The responses of AsegIR75q.1 to the two primary ligands at each dose were compared by independent samples t-test. ** in C indicates the doses at which the responses to octanoic acid were significantly different from the response to nonanoic acid (at 1 mM concentration, t = 9.055, d.f. = 8, two-sided p < 0.001; at 100 μM concentration, t = 4.486, d.f. = 8, two-sided p = 0.002)