Fig. 1

Nuclear cells (NCs) are able to crawl among the growing thrombi. A–C Thrombi (highlighted by red) with crawling NCs (highlighted by yellow) upon hirudin-anticoagulated blood perfusion through the flow chamber with fibrillar collagen fluorescent mode (DiOC6, A) or DIC (B, C) at × 100 magnification (raw data at https://doi.org/10.6084/m9.figshare.18356042.v1). D–G Thrombi with crawling NCs in the presence of DiOC6 (D), CD66b (E), and CD66ace (F) at × 20 magnification (G–merged D–F; raw data at https://doi.org/10.6084/m9.figshare.18417194.v1). H–K Among crawling CD66b and CD66ace positive cells were motionless cells with single nuclei (H) and clustered DiOC6 staining (I), which appeared to be CD2-positive (T lymphocyte marker; J) as well (K–merged H–J; raw data at https://doi.org/10.6084/m9.figshare.18435551.v1). L, M Different types of granulocytes were observed—crawling (L) and spread (M). O Granulocyte slowing down and spreading dynamics. O Based on the granulocyte and lymphocyte velocity distribution, it can be claimed that spread granulocyte and lymphocytes were distinctive not only by their appearance but by their velocity as well. Representative data out of N = 10 donors. Individual data values are given in the Additional Table 3