Fig. 3From: Ex vivo observation of granulocyte activity during thrombus formationAnnexin-V-positive platelets attach to the crawling granulocytes.A–C DiOC6 (A) and Annexin (B) staining colocalized (C) in a manner suggesting that granulocytes bear Annexin-V-positive platelets. D–F Similar conclusions can be made based on CD11b (D) and Annexin-V (E) colocalization (F) analysis. G, H Scatterplots of the Annexin-V and DiOC6 (G) or CD11b (H) fluorescence intensity correlation from C and F, correspondingly (raw data at https://doi.org/10.6084/m9.figshare.18613442.v1). I Pearson’s R correlation coefficient (PCC) for Annexin-V and DiOC6, CD11b, and CD66b dyes (n = 50 for each pair), individual data values are given in the Additional Table 3. J, K Microscopy images of the crawling granulocyte, bearing Annexin-V-positive platelets after 2 min (J) and after 9 min (K) from the start of the observation. L Trajectories of the granulocyte (green curve) and the Annexin-V-positive platelet (red curve) from J–K. Typical results out on n = 50 trajectoriesBack to article page