Fig. 4

The role of blood plasma proteins in NCs incorporation into the growing thrombi.A–F Confocal microscopy images of the growing thrombi and NCs. A, B Thrombi in the “clean” system on the collagen level (A) and 6 μm above (B). C, D Thrombi in the “clean” system with the addition of fibrinogen and VWF on the collagen level (C) and 6 μm above (D). E, F Thrombi in the whole blood on the collagen level (E) and 6 μm above (F). Representative images of n = 10 experiments (raw data could be found at https://doi.org/10.6084/m9.figshare.18358274.v1). G Scheme of the granulocyte behavior on the collagen covered glass coverslip (in 0.2 × 0.2 × 18 mm flow chamber): granulocytes (green cells) attach to the growing thrombi (yellow). In the presence of fibrinogen and VWF granulocytes, descend to the collagen level and collect Annexin-V-positive platelets from the thrombi in the process of crawling. Eventually, granulocytes slow down and spread on the collagen