Fig. 1

The dietary supplement of DHA prevents HFD-induced obesity. A Changes in body weight across time (n = 8). B Representative pictures of mice in three groups. C The food intake in three groups (n = 8). D Weight of subcutaneous adipose tissue (SAT) and epididymal white adipose tissue (eWAT) from mice in three groups, NS, not statistically significant (n = 3 or 4). E Basic serum triglyceride level of mice in three groups (n = 4). F Representative pictures of SAT and liver tissue from mice fed HFD and HFD+DHA. Scale bar: 200 μm. G Intraperitoneal glucose tolerance test (GTT) (n = 6), significant differences are shown between HFD and HFD+DHA (∗). H Area under curve (AUC) of GTT. I insulin tolerance test (ITT) (n = 6), significant differences are shown between HFD and HFD+DHA (∗∗). J AUC of ITT (n = 6). Statistical analysis was performed using one-way ANOVA with time-repeated measurements (Fig. 1A, G, and I), two-tailed paired Student’s t tests (Fig. 1C–E, H and J)