Fig. 7
From: DHA alleviates diet-induced skeletal muscle fiber remodeling via FTO/m6A/DDIT4/PGC1α signaling
FTO promotes PGC1α expression through DDIT4. A LC-MS/MS quantification of the m6A/A in mRNA of control, FTO-WT, and FTO-MUT overexpressing cells (n = 3). B Western blot analysis of FTO and PGC1α expression in control, FTO-WT, and FTO-MUT overexpressing cells (n = 3). C, D Methylated RNA immunoprecipitation (MeRIP)-qPCR analysis of m6A levels of Ppargc1a and Ddit4 mRNA in control and Fto-silencing cells (n = 3). E Western blotting analysis of FTO, DDIT4, and PGC1α in control and FTO knockdown cells (n = 3). F qPCR analysis of Ddit4 in control and FTO knockdown cells (n = 3). G Western blotting analysis of DDIT4 and PGC1α in control and DDIT4 knockdown cells (n = 3). H qPCR analysis of Ddit4 and Ppargc1a in control and DDIT4 knockdown cells (n = 3). Statistical analysis was performed using two-tailed paired Student’s t tests