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Fig. 1 | BMC Biology

Fig. 1

From: DIX domain containing 1 (DIXDC1) modulates VEGFR2 level in vasculatures to regulate embryonic and postnatal retina angiogenesis

Fig. 1

DIXDC1-KO mice showed delayed angiogenesis in embryonic and postnatal stage. A Whole-mount preparation of E10.5 embryo from WT and DIXDC1-KO mice immunostained for CD31. Blood vessel density and intersomitic vessel length decreased in DIXDC1-KO embryo compared to the WT. n = 5 per group. ICA, internal carotid artery; ISV, intersomitic vessels. Scale bars 500 μm. B, C Quantification of A (percentage of control). D Lateral view of unfixed E12.5 WT and DIXDC1 knockout embryo. n = 5 per group. E Quantification of D (percentage of control). F Whole-mount preparation of P6.5 retinae from WT and DIXDC1-KO pups immunostained for CD31. Radial length, vascular density, and number of filopodia decreased in DIXDC1-KO mice compared to the WT (individual white dots represent filopodia). n = 5 per group. Scale bars 500 μm and 50 μm. G, H, and I Quantification of radial length, number of tip cells per field, number of branches per field (percentage of control). All experiments were repeated on at least 3 different sets of WT and KO littermates. *p < 0.05, **p < 0.005, and ***p < 0.0001, by paired, 2-tailed Student’s t test. Error bars represent the mean ± SD. Individual values can be found in Additional file 6: Fig. 1

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